Tuesday, May 20, 2008

Why some like it spicy?   posted by Razib @ 5/20/2008 12:03:00 AM

Think Gene points me to a new PNAS paper, Structure of TRPV1 channel revealed by electron cryomicroscopy:
The transient receptor potential (TRP) family of ion channels participate in many signaling pathways. TRPV1 functions as a molecular integrator of noxious stimuli, including heat, low pH, and chemical ligands. Here, we report the 3D structure of full-length rat TRPV1 channel expressed in the yeast Saccharomyces cerevisiae and purified by immunoaffinity chromatography. We demonstrate that the recombinant purified TRPV1 channel retains its structural and functional integrity and is suitable for structural analysis. The 19-A structure of TRPV1 determined by using single-particle electron cryomicroscopy exhibits fourfold symmetry and comprises two distinct regions: a large open basket-like domain, likely corresponding to the cytoplasmic N- and C-terminal portions, and a more compact domain, corresponding to the transmembrane portion. The assignment of transmembrane and cytoplasmic regions was supported by fitting crystal structures of the structurally homologous Kv1.2 channel and isolated TRPV1 ankyrin repeats into the TRPV1 structure.

Think Gene and Scientific Blogging have summaries of the paper. Proteins are great, but what about the genes which produce them? I went to haplotter, and check out what I found....



Screenshot of genes around TRPV1


Also check out variation around that gene.

Related: Genetics of taste.